货号: A2910001
规格
Form: Liquid
Format: Bottle(s)
Cell Line: CHO-S, SFM Adapted
Product Size: 1000 mL
Classification: Animal Origin-Free, Protein-Free, Chemically Defined, Serum-Free
Shipping Condition: Room Temperature
内容及储存
Storage conditions: in the dark at 2-8°C
Shipping conditions: ambient
Shelf life: 12 months from date of manufacture
描述
ExpiCHO? Expression Medium is a GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin–free medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells. It is a core component of the ExpiCHO? Expression System Kit (Cat. No. A29133) forscalable transient protein production.
ExpiCHO Expression Medium:
? Supports growth of suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability
? Uses a transfection-compatible, serum-free suspension culture medium that enables high transfection efficiencies of >90% with
ExpiFectamine? CHO transfection reagent? Supports transient transfection of suspension CHO cells at a density of 6 x 106 cells per mL
? Facilitates sustained, high-level expression of high-density transiently transfected cultures, achieving yields of up to 3 g per L of
recombinant protein? Supports growth and transfection of CHO cells in culture formats from <1 ml="" in="" multi-well="" plates="" to="">10 L in benchtop bioreactors
? Does not contain phenol red
ExpiCHO Expression Medium is formulated with GlutaMAX?-I reagent. It is ready to use, with no supplementation required.The chemically-defined formulation results in high reproducibility and lot-to-lot reliability. For highest level transient protein expression, the medium is used in combination with a matched culture feed, ExpiCHO? Feed, which is supplied as part of the ExpiFectamine? CHO Transfection Kit (Cat. No. A29129). ExpiCHO Expression Medium is not recommended for adherent cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 ml="" in="" multi-well="" plates="" to="">10 L in bioreactors.
