Catalog Number:RHT03

CAS: 9002-07-7
EC: 3.4.21.4

Storage temperature: 2-8℃

Source: Recombinant human trypsin is a genetically engineered protein expressed in E.coli 

Protein Sequence:The amino acid sequence of recombinant human trypsin is identical to human pancreas-derived trypsin

1.DESCRIPTION

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant human trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence is identical to human pancreas-derived trypsin,with equivalent properties compared to native trypsin .The enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.

2.MAIN FEATURES

UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A₂₅₃ of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

3.RECOMMEND USAGE

Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.

4.STABILITY OF STORAGE AND TRANSPORT

• Stability of storage: Recombinant human trypsin lyophilized should be stored under 2-8℃ in sealed container.It is stable within 24 months.After dissolved with 1mM HCl , it should be stored under -20℃. It is no activity loss after 10 times repeated freezing and thawing.

• Stability of transport: The product is stable by blue ice insulation transport.

5.PRODUCT ADVANTAGE

• Animal origin free: Recombinant human trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.

• Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

• High purity: Higher specific activity.Host protein residues is less than the limits of biological products.

• Lyophilized powder: The product is lyophilized powder and is easy to store and transport.

• Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

• Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

6.PRODUCT USAGE

Recombinant human trypsin is an endopeptidase that can be used for lysine and arginine C-terminal cleavage of peptide bonds to cleave macromolecules into small peptides. Recombinant human trypsin is widely used in a variety of biotechnological processes, such as: cell culture cell separation of various tissues; degradation of denatured proteins; enzymatic hydrolysis, sequencing; stem cell therapy; tumor cell therapy.

7.RELATED PRODUCTS

Recombinant carboxypeptidase B

Sequencing grade trypsin

Recombinant trypsin

Recombinant enterokinase