Catalog Number:V813

CAS:66676-43-5

EC:3.4.21.19

Full Name:Sequencing grade recombinant V8 protease;Staphylococcus aureus strain V8

Synonyms:V8 protease;Endoproteinase Glu-C;Endoproteinase Asp-C 

Storage temperature: 2-8°C

Source: Recombinant V8 protease is a genetically engineered protein expressed in E.coli 

1. DESCRIPTION    

Recombinant V8 protease is the serine protease family and can specifically hydrolyzing glutamic acid or aspartic acid residues carboxy side peptide bonds. The Glu carboxy terminal peptide bond can be identified and cleaved in CH₄COONH₄ buffer (pH 7.8) and NH₄HCO₃ (pH 4.0 ).Identify and cleave the Glu or Asp carboxy terminal peptide bond in phosphate buffer (pH 7.8),and the hydrolysis rate of Glu was higher than that of Glu Asp. Recombinant V8 protease has activity between pH 4.0-10.0, and the optimum pH is 8.0-8.5. It can be used alone or in combination with other proteases when used in protein digestion, peptide profiling and peptide mass fingerprinting analysis. The inhibitors of V8 protease are diisopropylfluorophosphate (DFP), α2-macroglobulin and Nα-P-tosyl-L-lysine chloromethyl ketone (TLCK).

2. MAIN FEATURES

UNIT DEFINITION: One unit is defined as the amount of enzyme required to catalytic substrate Z-Phe-Leu-Glu-4-nitranilide produced 1 μmol of 4-nitroaniline per minute at 25°C and pH 7.8 .

3. STABILITY OF STORAGE AND TRANSPORT

（1）Stability of storage: Recombinant V8 protease should be stored under 2-8°C in sealed container.It is stable within 6 months after dissolved with1mM HCl and 50mM HAC at -20°C or below. It is stable within 2 months after dissolved with 1mM HCl and 50mM HAC at 4°C.85% activity above can be kept after dissolved with 1mM HCl and 50mM HAC at 25°C or 37°C 12h. It is no activity loss after 10 times repeated freezing and thawing.

（2）Stability of transport: The product is stable by blue ice insulation transport.