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产地 | |
品牌 | life |
货号 | 12183020 |
用途 | |
英文名称 | |
包装规格 | |
纯度 | % |
CAS编号 | |
保质期 | 6个月 |
是否进口 | 是 |
货号: 12183020
Sample Type (General): | Bacteria, Yeast, Blood, Tissue, Plant Samples, Cells |
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High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
Downstream Application: | Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction, Nuclease Protection Assays, Next-Generation Sequencing, Microarray Analysis, Real-Time Quantitative PCR (qPCR) |
Scale: | Mini |
System: | PureLink™ |
Quantity: | 10 columns |
Product Size: | 10 preps |
Final Product: | Total RNA |
Yield (up to): | 1000 μg |
Number of Reactions: | 10 Preps |
Elution Volume - max: | 300 μl |
Elution Volume - min: | 30 μl |
Isolation Technology: | Silica Spin Column |
Starting Material (Amount): | Up to 200 mg tissue, 5 x 10^6 to 1 x 10^8 cells |
Shipping Condition: | Room Temperature |
The PureLink® RNA Mini Kit comes with enough RNA lysis solution, spin cartridges, wash tubes, recovery tubes, wash buffers, and RNase-free water to perform 10 RNA purifications. All components should be stored at room temperature. Guaranteed stable for 1 year when properly stored.
The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. The PureLink® RNA Mini Kit provides:
? Rapid, non-hazardous RNA purification capabilities
? Ready-to-use RNA for many downstream applications
Rapid and convenient column purification
The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1,000 μg of purified RNA from a single extraction (see table). High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.
Simplified, nontoxic RNA purification
The PureLink® RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.