|
| 产地 | |
| 品牌 | life |
| 货号 | 100 mL |
| 用途 | |
| 英文名称 | |
| 包装规格 | |
| 纯度 | % |
| CAS编号 | |
| 保质期 | 6个月 |
| 是否进口 | 是 |
货号： 12604013
| Chelators: | EDTA |
|---|---|
| Phenol Red Indicator: | No Phenol Red |
| Classification: | Animal Origin-Free |
| Enzyme Stability: | Room Temperature Stable |
| Form: | Liquid |
| pH Range: | Check and Record |
| Osmolality: | Check and Record |
| Concentrated: | 1 X |
| Product Size: | 100 mL |
| Reagent Type: | Trypsin Replacement |
| Tests Performed: | In Vitro Bioassay |
| Green Features: | Energy efficient, Sustainable packaging, Less hazardous |
| Shipping Condition: | Room Temperature |
描述
TrypLE™ Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE™ Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. Life Technologies offers a variety of TrypLE™ formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, TrypLE™ Express is:
? Gentle on cells
? Room temperature-stable
? Easy to use
? Animal origin-free (AOF)
This TrypLE™ Express is modified as follows:
| With | Without |
| ? EDTA | ? Phenol Red |
The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.
Gentle on cells
TrypLE™ Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.
Room temperature-stable
TrypLE™ Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.
Easy to use
TrypLE™ Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Express, avoiding the need for trypsin inhibitors, such as FBS.
Animal origin-free (AOF)
Unlike porcine trypsin, TrypLE™ Express is free of animal-derived components.
cGMP manufacturing and quality system
TrypLE™ Express is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
