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| 产地 | |
| 品牌 | Essential |
| 货号 | A1516401 |
| 用途 | |
| 英文名称 | |
| 包装规格 | 500 mL |
| 纯度 | % |
| CAS编号 | |
| 保质期 | 6个月 |
| 是否进口 | 否 |
货号： A1516401
| Cell Type: | Stem Cells (iPS - Induced Pluripotent Stem) |
|---|---|
| Form: | Liquid |
| Species: | Human |
| Quantity: | 1 bottle(s) |
| Serum Level: | Serum-Free |
| Culture Type: | Feeder Cell-Free |
| Product Size: | 500 mL |
| Classification: | Xeno-Free |
| Volume (Metric): | 500 ml |
1 × 500 ml bottle, Store at 2 to 8°C. Protect from light.
Essential 6™ Medium is a feeder-free and xeno-free medium that supports the reprogramming of somatic cells and differentiation of human pluripotent stem cells (PSCs). The formulation is based on a medium originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson and published as "E8." Essential 6™ Medium has been proven to support somatic cell reprogramming using a variety of methods, including episomal vectors (2) and CytoTune™ (Sendai virus). In addition, Essential 6™ Medium can be used as a base for the formulation of custom media for the culture of PSCs.
Essential 6™ Medium enables:
? Reprogramming—does not contain TGFΒ, which has a negative effect on reprogramming efficiency
? Differentiation—does not contain bFGF, which inhibits embryoid body formation and differentiation
? Flexibility—provides a flexible format where the levels of TGFΒ and bFGF can be adjusted and additional components can be added to match a given application
Reprogramming
Essential 6™ Medium allows for defined and feeder-free reprogramming when used with bFGF. The formulation supports somatic cell reprogramming using a variety of methods, including episomal vectors (2) and CytoTune™ (Sendai virus), and is optimized to help ensure maximum cell health and pluripotency with minimal variability.
Differentiation
Essential 6™ Medium is a reliable and robust medium for the formation of embryoid bodies. Unlike other media used in PSC culture, Essential 6™ Medium does not contain bFGF or TGFβ, which inhibits embryoid body formation and differentiation.
Flexibility
Essential 6™ Medium is xeno-free and contains only the essential components needed for stem cell culture, minus bFGF and TGFΒ. This provides a basal medium that will ensure maximum cell health and pluripotency, while allowing levels of TGFΒ and bFGF to be adjusted and additional components to be added to match a given application.
