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| 产地 | |
| 品牌 | GeneArt |
| 货号 | A21174 |
| 用途 | |
| 英文名称 | |
| 包装规格 | |
| 纯度 | % |
| CAS编号 | |
| 保质期 | 6个月 |
| 是否进口 | 否 |
货号： A21174
| Promoter: | CMV, U6 |
|---|---|
| Reporter: | OFP |
| Key Function: | Episomal Expression, Genome Engineering, Gene Editing, Stable Cell Line Development |
| Product Size: | 10 reactions |
| Cloning Method: | Restriction Enzyme ? MCS |
| Delivery Method: | Transfection |
| Protein Tag or Fusion: | OFP fusion |
Contains:
? CRISPR OFP Nuclease Vector, linearized
? 10X Oligonucleotide Annealing Buffer
? DNase/RNase-free Water
? 5X Ligation Buffer
? T4 DNA Ligase
? U6 Forward Sequencing Primer
? Control Double-stranded Oligonucleotide
Store in freezer (-5 to -30°C).
The GeneArt® CRISPR Nuclease Vector with OFP Reporter Kit is a vector system for expression of the functional components needed for CRISPR/Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter. The OFP reporter allows for fluorescence-based tracking of transfection efficiency, as well as FACS-based sorting/enrichment of Cas9 and CRISPR expressing cells. The linearized GeneArt® CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease.
The GeneArt® CRISPR Nuclease Vector system offers:
? Easy-to-design genome engineering system
? Affordable, ready-to-use cloning vectors
? Enrichment for hard to transfect or difficult to modify cell lines
All-in-one Vector System for CRISPR-based Genome Editing
The GeneArt® CRISPR Nuclease Vector kit offers a simple, ready-to-use, all-in-one expression vector system consisting of both a Cas9 nuclease expression cassette and a guide RNA (gRNA) cloning cassette for rapid and efficient cloning of DNA that encodes target-specific CRISPR RNA (crRNA). This system allows you to edit and engineer a genomic locus of choice in a sequence-specific manner from a single plasmid. After relevant targets have been identified with fast and easy-to-use GeneArt® CRISPR vectors, the biologically relevant mutations can be precisely created with GeneArt® Precision TALs, with high specificity and low off-target effects.
