• 商品编号：304-295-003

• 品牌：Jackson

• 公司：Jackson ImmunoResearch Laboratories

• 公司分类：Whole IgG Affinity-Purified Antibodies

• Target：Dog

• Host：Rabbit

• Specificity：IgG (H+L)

• Conjugate：Rhodamine Red™-X (RRX)

• Unit：1.5 mg

• Antibody_Format：Whole IgG

• Clonality：Polyclonal

• 产地：美国

WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.

Basedonimmunoelectrophoresisand/orELISA ,theantibodyreactswithwholemoleculedogIgG.Italsoreactswiththelightchainsofotherdogimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.Theantibodymaycross-reactwithimmunoglobulinsfromotherspecies.

PhysicalState:Freeze-driedsolid
Storage:

Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing.
Expirationdate:oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.

Purity:Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.
Buffer:0.01MSodiumPhosphate,0.25MNaCl,pH7.6
Preservative:0.05%SodiumAzide

SuggestedWorkingConcentrationorDilutionRange:
Histo-/Cyto-Chemistry:-1:50-1:200
FlowCytometry:-1:50-1:200

Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeABI lity,etc.Theactualdilutionusedmustbedeterminedempirically.

RhodamineRed™-X(RRX)

Amax:570Emax:590nm

RRX(RhodamineRed-X)conjugateshaveapeakofexcitationat570nmandapeakofemissionat590nm.AlthoughTRITChasbeenusedtrADI tionallywithFITCfordoublelabeling,bettercolorseparationisachievedbyusingRRXorAlexaFluor®594.RhodamineRed-Xisparticularlyusefulfor3-and4-colorlabelingwithDyLight405,AlexaFluor®488,andAlexaFluor®647byusingaconfocalmicroscopeequippedwitha405nmlaserandakrypton/argonlaser.FluorescencefromRRXliesaboutmidwaybetweenthatofAlexaFluor®488andAlexaFluor®647,anditshowslittleoverlapwitheitherdye.Thekrypton-argonlaseremitslinesat488nm,568nm,and647nm,whichareoptimalforexcitingAlexaFluor®488,RRX,andAlexaFluor®647,respectively.Byaddinga405nmlaseranda420nmemissionfilter,4-colorlabelingispossIBL eusingDyLight405-conjugatedsecondaryantibodiesfromJIR(Figure5).Theseparationbetweenallfourdyesisperfectfor4-colorlabeling,andallfourdyesareverybright.