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| 产地 | |
| 品牌 | GoldBio |
| 货号 | C-529-50 |
| 用途 | |
| 英文名称 | |
| 包装规格 | 50 μl |
| 纯度 | % |
| CAS编号 | |
| 保质期 | 一年 |
| 是否进口 | 是 |
商品编号：C-529-50
品牌：GoldBio
公司：Gold Biotechnology, Inc.
公司分类：ENZYMES, INHIBITORS & SUBSTRATES
Size：50 μl
Cas9NucleaseisthepurifiedrecombinantStreptococcuspyogenesCas9enzymecontaininganuclearlocalizationsignal(NLS)attheC-terminalfortargetingtothenucleus.ThisenzymeisdesignedtoperformCRISPR/Cas9-mediatedgenomeediting.Thephysicalpurityofthisenzymeis≥98%asassessedbySDS-PAGEwithCoomassiebluestaining.
CRISPR(Clusteredregularlyinterspacedshortpalindromicrepeats)/CRISPR-associated(Cas)systemsprovidebacteriaandarchaeawithadaptiveimmunityagainstvirusesandplasmidsbyusingCRISPRRNAs(crRNAs)toguidethesilencingofinvADI ngnucleicacids.TheCRISPRsystemconsistsofashortnon-codingguideRNA(sgRNA)madeupofatargetcomplementaryCRISPRRNA(crRNA)andanauxiliarytransactivatingcrRNA(tracrRNA).ThesgRNAguidestheCas9endonucleasetoaspecificgenomiclocusviabasepairingbetweenthecrRNAsequenceandthetargetsequence,andcleavestheDNAtocreateadouble-strandbreak.Thelocationofthebreakiswithinthetargetsequence3basesfromtheNGGPAM(ProtospacerAdjacentMotif).ThePAMsequence,NGG,mustfollowthetargetedregionontheoppositestrandoftheDNAwithrespecttotheregioncomplementarysgRNAsequence.
Cas9nucleaseisfreefromdetectableRNase,Endonuclease(nicking)andnon-specificDNaseactivities.
ProductIncludes
Cas9Nuclease(1600ng/μl(10μM))
10xCas9NucleaseReactionBuffer
Source:E.coliBL21(DE3)strainexpressingaCas9genefromStreptococcuspyogeneswithanN-terminal6xHistagandC-terminalSV40nuclearlocalizationsignal(NLS)
Storage/Handling:Storeat-20°C.
Productmaybeshippedonblueice.
