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产地 | |
品牌 | 国产 |
货号 | |
用途 | 科研实验 |
英文名称 | CoraLite®488-Conjugated Alpha Tubulin Monoclonal Antibody |
包装规格 | |
纯度 | % |
CAS编号 | |
保质期 | 一年 |
是否进口 | 否 |
Applications:
WB, IF, ELISA
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E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite®488-conjugated version of this antibody, CL488-66031 (alpha Tubulin antibody), at dilution of 1:100.
Various lysates were subjected to SDS PAGE followed by western blot with CL488-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Species specificity:
human, mouse, rat, dog
Positive WB detected in:
HepG2 cells, HeLa cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF detected in:
HeLa cells, mouse embryo tissue
Recommended dilution:
WB : 1:300-1:600
IF : 1:50-1:500
Excitation/Emission maxima wavelengths:
488 nm/515 nm