QuikChange II

The second generation of our QuikChange method that provides improved fidelity over our original kit, while maintaining greater than 80% mutation efficiency for single site mutagenesis.

• Includes PfuUltra High-Fidelity DNA Polymerase to minimize unwanted errors

• Complete kit provides all reagents necessary to support mutagenesis (at single sites), for large constructs, and for use with electroporation competent cells.

• Free access to Primer Design Program

• QuikChange Lightning Live Webinar: Accelerate your research without sacrificing mutation efficiency, reliability and accuracy. Click here to view   

For Research Use Only. Not for Use in Diagnostic Procedures.

QuikChange II Site-Directed Mutagenesis Kits - Details & Specifications

The QuikChange II site-directed mutagenesis kit is used to make point mutations, replace amino acids, and delete or insert single or multiple adjacent amino acids. The QuikChange II site-directed mutagenesis method is performed using PfuUltra high-fidelity (HF) DNA polymerase for mutagenic primer-directed replication of both plasmid strands with the highest fidelity. The basic procedure utilizes a supercoiled double-stranded DNA (dsDNA) vector with an insert of interest and two synthetic oligonucleotide primers, both containing the desired mutation (see Figure 1). The oligonucleotide primers, each complementary to opposite strands of the vector, are extended during temperature cycling by PfuUltra HF DNA polymerase, without primer displacement. Extension of the oligonucleotide primers generates a mutated plasmid containing staggered nicks. Following temperature cycling, the product is treated with Dpn I. The Dpn I endonuclease (target sequence: 5′-Gm6ATC-3′) is specific for methylated and hemimethylated DNA and is used to digest the parental DNA template and to select for mutation-containing synthesized DNA.6 (DNA isolated from almost all E. coli strains is dam methylated and therefore susceptible to Dpn I digestion.) The nicked vector DNA containing the desired mutations is then transformed into competent cells.

Figure 1. Overview of the QuikChange II Site-directed Mutagenesis Method.

QuikChange II Site-Directed Mutagenesis Kit

For short (4kb – 8kb) targets.  Includes XL1-Blue Competent Cells.

QuikChange II XL Site-Directed Mutagenesis Kit

For long (8 kb – 14kb) or difficult targets.  Includes XL10-Gold Ultracompetent Cells.

QuikChange II-E Site-Directed Mutagenesis Kit

For researchers performing mutagenesis via transformation into electroporation-competent cells.  Includes XL1-Blue Electroporation-competent Cells.

QuikChange II XL定点诱变试剂盒

• 优化后用于大的>8kb和难操作的的质粒

• 比基于PCR突变更精确

• 包含PfuUltra高保真DNA聚合酶，以*程度地减少无用的错误

          QuikChange II XL定点诱变试剂盒是专为大的或难以操作的靶基因的成功突变而设计。试剂盒包括了PfuUltra DNA聚合酶，QuikSolution试剂，和XL10-Gold超级感受态细胞，以保证您在单日里成功诱变大的构建。PfuUltra聚合酶轻松地扩增大的质粒和给予高精确性，可以*程度降低次位点突变的几率，添加QuickSolution试剂到您的诱变反应更进一步改进大的和难操作模板的扩增。XL10-Gold超级感受态细胞以*效率性转化大的DNA分子。

QuikChange II XL定点诱变试剂盒

内含物：PfuUltra高保真DNA聚合酶，QuickSolution试剂，Dpn I限制性内切酶，10×反应缓冲液，QuikChange质控质粒和质控引物，dNTPs混合，XL10-Gold超级感受态细胞，XL10-Gold BME，pUC18质控质粒