Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A32732) in WB
Western blot analysis of Heat Shock Protein 90 (Hsp90) was performed by loading 3-fold serial dilutions of A431 whole cell lysate (starting at 15 μg) and 2 μL of the PageRuler Prestained NIR Protein Ladder (Product # 26635 ) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to Nitrocellulose Membranes (Product # 88018 ) and blocked with Fluorescence Blocker for 30 min. Membranes wer... View more
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