Invitrogen™ Zenon pHrodoiFL IgG Labeling Reagents
- 价格： ￥10/瓶
- 发布日期： 2021-05-27
- 更新日期： 2025-04-30
产品详请
| 产地 |
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| 品牌 |
Invitrogen
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| 货号 |
Z25611
|
| 用途 |
科研实验
|
| 英文名称 |
|
| 包装规格 |
|
| 纯度 |
%
|
| CAS编号 |
|
| 保质期 |
一年
|
| 是否进口 |
是
|
Zenon™ pHrodo™ iFL Green-labeled or Red-labeled Fab fragments (i.e. labeling reagent) are directed against the Fc portion of an intact IgG primary antibody to form a labeling complex. Formation of the Fab–antibody complex occurs in fewer than 5 minutes. Because the pHrodo™ iFL dyes dramatically increase in fluorescence as the pH of their surroundings becomes more acidic, the antibodies coupled with the Zenon™ pHrodo™ iFL IgG Labeling Reagents provide an excellent measure of endocytic activity based on the acidification of the labeled antibodies as they are ingested by the cell.
The Zenon™ pHrodo™ iFL IgG Labeling Reagents contain sufficient material to label one to four 96-well plates, depending on the Zenon™ labeling reagent concentration used. Only a small amount of starting material is needed, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions.
Other advantages of the pH-sensitive Zenon™ pHrodo™ iFL IgG Labeling Reagents include:
? Simplicity &mdash No reagent purification is required
? Accuracy &mdash No wash steps or quenchers are needed, minimizing cross-reactivity or background
? Speed &mdash Labeled antibodies are typically ready to use in 10 minutes
? Specificity &mdash Dyes are designed for the specific detection of phagocytosis and endocytosis
