Workflow of CellROX® Orange Reagent

Fluorescence Excitation and Emission Spectra of the Oxidized CellROX® Orange Reagent

Detection of Oxidative stress using CellROX® orange and flow cytometer

Human osteosarcoma (U2-OS) cells expressing CellLight?®Lysosomes-GFP (catalog # C10507) were treated with 200 ?μM tert-Butyl hydroperoxide for 2 hr. A stain solution containing 5 ?μM CellROX?® Orange (Catalog # C10443) and 2 drops/ml of NucBluea?￠ Live Cell Stain (Catalog # R37605) was applied for 30 min at 37?°C. Cells were washed and imaged with Live Cell Imaging Solution (catalog # A14291DJ).

Oxidative stress detection in Human osteosarcoma (U2-OS) cells:

Oxidative stress detection in Human osteosarcoma (U2-OS) cells:

Nefazodone Induced Oxidative Stress Measured with CellROX™ Orange Reagent in HepG2 Cells

Tert-butyl Hydroperoxide (TBHP) Induced Oxidative Stress Measured with CellROX™ Orange Reagent in Bovine Pulmonary Endothelial Cells (BPAE)

Oxidative stress detection in Human osteosarcoma (U2-OS) cells:

Oxidative stress detection in Human osteosarcoma (U2-OS) cells:

Oxidative stress detection in Human osteosarcoma (U2-OS) cells:

Oxidative stress detection in Human osteosarcoma (U2-OS) cells:

Oxidative stress detection in Human osteosarcoma (U2-OS) cells:

Oxidative stress detection in Human osteosarcoma (U2-OS) cells:

Oxidative stress detection in Human osteosarcoma (U2-OS) cells:

Angiotensin II Induced Oxidative Stress Measured with CellROX™ Orange Reagent in Human Aortic Smooth Muscle Cells

Oxidative stress detection in Human osteosarcoma (U2-OS) cells:

Tert-butyl hydroperoxide (TBHP) Induced Oxidative Stress Measured with CellROX™ Orange Reagent in Bovine Pulmonary Endothelial Cells (BPAE)

Detection of Oxidative stress using CellROX® orange and Tali® Image-Based Cytometer

Oxidative stress detection in Human osteosarcoma (U2-OS) cells: