描述

Applied Biosystems® TaqMan® Protein Expression Assays enable fast, easy identification, and relative protein quantification of pluripotency protein markers from limited quantities of cultured hESCs. The TaqMan® Protein Expression Assays Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan® protein expression assays. The Universal PCR Assay contains optimized concentrations of TaqMan® probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems® real-time PCR system. The enzyme concentration, ionic strength, and the pH of the TaqMan® Protein Expression Fast Master Mix are optimized for the amplification of ligated oligonucleotide target sequences prepared using TaqMan® Protein Expression Assay reagents. The DNA polymerase included in the master mix is designed for maximum PCR efficiency and specificity when used with the ligation chemistry. Key features of the TaqMan® Protein Expression Assay: 
? Quantitates protein expression using gold standard TaqMan® 5’ nuclease chemistry 
? Enables direct correlation of mRNA and/or miRNA expression to protein expression on the same real-time PCR platform 
? Allows greater sensitivity and a more quantitative readout than western blots and immunostaining.

规格

产品线:	TaqMan™
技术:	Proximity Ligation Assay
热启动:	No
参比荧光染料:	ROX (Pre-mixed)
聚合酶:	AmpliTaq™ Fast DNA Polymerase
引物探针兼容性:	TaqMan™
PCR 方法:	qPCR Protein Quantification
纯度或质量等级:	UP (Ultra Pure)
反应速度:	Fast
样品类型:	Cells
靶标特异性:	Not Target-Specific
检测方法:	Primer-Probe Detection
适用于（设备）:	7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
标签或染料:	FAM

内容及储存