北京批发Sigma C9263CollagenaseⅤ 胶原酶Ⅴ价格

Analysis NoteAlso contains clostripain, nonspecific neutral protease and tryptic activities.
Unit DefinitionOne collagen digestion unit liberates peptides from collagen equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hr at pH 7.4 at 37 °C in the presence of calcium ions.
One FALGPA Hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 °C in the presence of calcium ions.
One Neutral Protease Unit hydrolyzes casein to produce color equivalent to 1.0 μmole tyrosine per 5 hr at pH 7.5 at 37 °C.
One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of DTT.
 

TESTSPECIFICATION
appearancereport result
collagen digestion activityNLT 125 units/mg solid
unit definitionOne unit liberates peptides from collagen （C9879） equivalent in ninhydrin color to 1.02 micromole of leucine in 5hours at pH7.4 at 37degc in the presence of calcium ions.
falgpa hydrolysis activity1 to 3 units/mg solid
unit definitionOne unit hydrolyzes 1.0micromole of falgpa （furylacryloyl-leu-gly-pro-ala, F-5135） per minute at pH7.5 at 25degc in the presence of calcium ions.
caseinase activityreport result
clostripain activityreport result
suitability test for cell releasetested for preparation of islets from rat pancreas by a modification of the method of lacy & kostianovsky, diabetes, 16, p35（1967）

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