高品质Sigma L-乳酸脱氢酶（ L2500）价格

L-Lactic Dehydrogenase from rabbit muscle Type II, ammonium sulfate suspension, 800-1,200 units/mg protein
Synonym （S）-Lactate: NAD+ oxidoreductase
L-LDH
LAD
LD
CAS Number 9001-60-9
 
Descriptions
Analysis Note ………………………..Protein determined by biuret.
Biochem/physiol Actions …. ................Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
Unit Definition ……………………….One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
Physical form ………………………..Crystalline suspension in 3.2 M （NH4）2SO4 solution, pH 6.0
General description ………………….Lactic Dehydrogenase （LDH） has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit （muscle） and H subunit （heart）. These subunits may be mixed in any of 5 combinations （M4, M3H1, M2H2, MH3, and H4）. Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species （MW = ~70 kDa） in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 （1974）. Oxidizes glyoxylate and lactate.
Isoelectric point: 8.4-8.6
Optimal pH : 7.5 .
Properties
type ………………………………….Type II
form …………….……………………ammonium sulfate suspension
foreign activity………...………........….pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase <0.01%
storage temp. 2-8°C
 

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