促销Sigma Glucose oxidase 葡萄糖氧化酶（G7141）说明书

品名称葡萄糖氧化酶 ： Glucose Oxidase from Aspergillus niger
CAS编号9001-37-0
别名葡糖氧化酶，β-D-Glucose:oxygen 1-oxidoreductase G.Od. GOx
级别科研级
纯度&分析Type X-S, lyophilized powder, 100,000-250,000 units/g solid （without added oxygen）
Tips来源于黑曲霉。
MDL编号MFCD00131182
EINECS编号232-601-0
Beilstein编号N/A
PubChem编号N/A
分子式N/A
分子量N/A
储存条件-20 °C 低温冷冻保存。
性质typeType X-S
formlyophilized powder
compositionProtein, 65-85%
foreign activityCatalase ≤5 units/mg protein
storage temp.−20°C

原产地美国
描述Analysis Note
Protein determined by biuret.
Application
Glucose oxidase is widely used in the food and pharmaceutical industries1 as well as a major component of glucose biosensors.2
Biochem/physiol Actions
Glucose oxidase catalyses the oxidation of β-d-glucose to d-glucono-β-lactone and hydrogen peroxide, with molecular oxygen as an electron acceptor.3,4
Quality
May contain traces of amylase, maltase, glycogenase, invertase, and galactose oxidase.
Unit Definition
One unit will oxidize 1.0 μmole of β-D-glucose to D-gluconolactone and H2O2 per min at pH 5.1 at 35 °C, equivalent to an O2 uptake of 22.4 μl per min. If the reaction mixture is saturated with oxygen, the activity may increase by up to 100%.
General description
Molecular Weight: 160 kDa （gel filtration）
pI: 4.2
Extinction coefficient: E1% = 16.7 （280 nm）

Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for N-linked glycosylation.

Glucose oxidase is capable of oxidizing D-aldohexoses, monodeoxy-D-glucoses, and methyl-D-glucoses at varying rates.

The pH optimum for glucose oxidase is 5.5, while it has a broad activity range of pH 4-7. Glucose oxidase is specific for β-D-glucose with a KM of 33-110 mM.

Glucose oxidase does not require any activators, but it is inhibited by Ag+, Hg2+, Cu2+, phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide.

Glucose oxidase can be utilized in the enzymatic determination of D-glucose in solution. As glucose oxidase oxidizes β-D-glucose to D-gluconolactate and hydrogen peroxide, horseradish peroxidase is often used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for β-D-glucose, solutions of D-glucose can be quantified as α-D-glucose will mutorotate to β-D-glucose as the β-D-glucose is consumed by the enzymatic reaction.
供货状态True
参考文献1. W.M. Fogarty & C.T. Kelly, Eds,, Elsevier Applied Science Microbial Enzymes and Biotechnology, 177-226, （1990）
2. A.C. Ayupe de Oliveira et al., Flow-injection system with glucose oxidase immobilized on a tubular reactor for determination of glucose in blood samples Anal. Chim. Acta 535, 213-217, （2005）
3. J. Rogalski et al., Optimization of glucose oxidase synthesis in submerged cultures of Aspergillus niger G-13 mutant Enzyme Microb. Technol. 10, 508-511, （1988）
4. T. Lu et al., The production of glucose oxidase using the waste myceliums of Aspergillus niger and the effects of metal ions on the activity of glucose oxidase Enzyme Microb. Technol. 19, 339-342, （1996）
Rzygalinski, I., et al., Enzymatic in-capillary derivatization for glucose determination by electrophoresis with spectrophotometric detection. Electrophoresis 29, 1741-8, （2008）
Kudo, H., et al., Glucose sensor using a phospholipid polymer-based enzyme immobilization method. Anal. Bioanal. Chem 391, 1269-74, （2008）
Cole, N.B., et al., Oxidative Stress Causes Reversible Changes In Mitochondrial Permeability And Structure. Exp. Physiol. 45, 596-602, （2010）
A. Crueger, W. Crueger, Glucose transforming enzymes Microbial Enzymes and Biotechnology, （1990）, 177-226
Merck 14,4460
类型酶 & 辅酶

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