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CAS
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300-08-3
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中文名称
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氢溴酸槟榔碱
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英文名称
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Arecoline Hydrobromide
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别名
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氢溴酸槟榔碱;Arecoline HBr;Arecoline bromide
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纯度
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HPLC≥98%
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分子式
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C8H13NO2·HBr
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分子量
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236.11
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外观（性状）
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White to off-white Solid
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储存条件
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Powder : 2-8℃, 2 years; In solvent(母液): -20℃, 1 month; -80℃, 6 months
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溶解性
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Soluble in Water/DMSO ≥5mg/mL
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MDL
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MFCD00039041
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EC
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EINECS 206-087-3
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InChIKey
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AXOJRQLKMVSHHZ-UHFFFAOYSA-N
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InChI
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InChI=1S/C8H13NO2.BrH/c1-9-5-3-4-7(6-9)8(10)11-2;/h4H,3,5-6H2,1-2H3;1H
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PubChem CID
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9301
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SMILES
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O=C(C1=CCCN(C)C1)OC.Br
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描述
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是毒蕈碱乙酰胆碱受体(mAChR)激动剂。(It is a muscarinic acetylcholine receptor (mAChR) agonist.)
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靶点
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mAChR
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通路
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Neuronal Signaling
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生物活性
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Arecoline, an arecanut alkaloid present in the saliva of betel quid chewers, has been implicated in the pathogenesis of a variety of inflammatory oral diseases, including oral submucous fibrosis and periodontitis.[1] A partial agonist of nicotinic and muscarinic acetylcholine receptors, arecoline evokes multiple effects on the central nervous system (CNS), including stimulation, alertness, elation, and anxiolysis. [2]
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In Vitro
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Arecoline induced the generation of reactive oxygen species and cell cycle arrest at the G1/G0 phase in HaCaT cells without affecting the expression of p21/Cip1. Arecoline-induced epithelial cell death at higher concentrations was caused by oxidative trauma without eliciting apoptosis. Sublethal concentrations of arecoline upregulated the expression of the following stress-responsive genes: heme oxygenase-1; ferritin light chain; glucose-6-phosphate dehydrogenase; glutamate-cysteine ligase catalytic subunit; and glutathione reductase. Additionally, there was a dose-dependent induction of interleukin-1alfa mRNA by arecoline via oxidative stress and p38 MAPK activation.[1]
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细胞实验
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Human keratinocyte cells (of the HaCaT cell line) were treated with arecoline, following which cell viability was assessed using the Trypan Blue dye-exclusion assay, cell growth and proliferation were analyzed using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) and 5-bromo-2-deoxyuridine incorporation assays, cell cycle arrest and generation of reactive oxygen species were examined using flow cytometry, and gene expression changes were investigated using the reverse transcription-polymerase chain reaction technique.[1]
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数据来源文献
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[1]. Thangjam GS, et, al. Regulation of oxidative-stress responsive genes by arecoline in human keratinocytes. J Periodontal Res. 2009 Oct;44(5):673-82.
[2]. Volgin AD, et, al. DARK Classics in Chemical Neuroscience: Arecoline. ACS Chem Neurosci. 2019 May 15;10(5):2176-2185.
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规格
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50mg 100mg 10mM*1mL (in DMSO)
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单位
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瓶
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