|
CAS
|
76823-03-5
|
|
中文名称
|
5-羧基荧光素
|
|
英文名称
|
5-FAM
|
|
别名
|
5-Carboxyfluorescein
|
|
纯度
|
HPLC≥95%
|
|
分子式
|
C21H12O7
|
|
分子量
|
376.32
|
|
外观（性状）
|
Light yellow to yellow Solid
|
|
储存条件
|
Powder : 2-8℃, 2 years;In solvent(母液): -20℃, 1 month; -80℃, 6 months
|
|
溶解性
|
Soluble in DMSO
|
|
EC
|
EINECS 276-331-1
|
|
MDL
|
MFCD00036874
|
|
InChIKey
|
NJYVEMPWNAYQQN-UHFFFAOYSA-N
|
|
InChI
|
InChI=1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
|
|
PubChem CID
|
123755
|
|
SMILES
|
O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=C(C(O)=O)C=C5
|
|
描述
|
5-FAM是羧基荧光素的一个同分异构体。(5-FAM is an isomer of carboxyfluorescein.)
|
|
In Vitro
|
5-FAM contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity， excellent fluorescence quantum yield， and good water solubility， fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser[1].
|
|
数据来源文献
|
[1]. Jungmi Lee, et al. Peptide substrate-based inkjet printing high-throughput MMP-9 anticancer assay using fluorescence resonance energy transfer (FRET). Sens Actuators B Chem. 2018 Mar;256:1093-1099.
|
|
规格
|
10mg 10mM*1mL (in DMSO) 50mg
|
|
单位
|
瓶
|
